TY - JOUR T1 - Homogeneous Line Width of the Different Vibronic Bands of Retinal Absorption in Bacteriorhodopsin by the Hole-Burning Technique JF - The Journal of Physical Chemistry Y1 - 1996 A1 - Kamalov, Valey F. A1 - Masciangioli, Tina M. A1 - El-Sayed, Mostafa A AB - Using the hole-burning technique, resolved vibrational structure was observed in the retinal absorption spectrum of bacteriorhodopsin (bR) in poly(vinyl alcohol) (PVA) film at 10 K with 556 and 632 nm irradiation. The homogeneous line widths of v = 1 and v = 2 vibronic bands are estimated from the deconvolution of the observed spectrum. The absorption maximum is found to shift by 100?200 cm-1 by using the two excitation wavelengths; resulting from partial site selection due to the contribution of inhomogeneous broadening. The hole width produced by excitation near the zero-phonon band is found to be ?1250 cm-1, which corresponds to a homogeneous width of ?600 cm-1, and the low limit of dephasing time can be estimated as 20 fs. This width is found to be independent of the vibronic band observed.Using the hole-burning technique, resolved vibrational structure was observed in the retinal absorption spectrum of bacteriorhodopsin (bR) in poly(vinyl alcohol) (PVA) film at 10 K with 556 and 632 nm irradiation. The homogeneous line widths of v = 1 and v = 2 vibronic bands are estimated from the deconvolution of the observed spectrum. The absorption maximum is found to shift by 100?200 cm-1 by using the two excitation wavelengths; resulting from partial site selection due to the contribution of inhomogeneous broadening. The hole width produced by excitation near the zero-phonon band is found to be ?1250 cm-1, which corresponds to a homogeneous width of ?600 cm-1, and the low limit of dephasing time can be estimated as 20 fs. This width is found to be independent of the vibronic band observed. PB - American Chemical Society VL - 100 SN - 0022-3654 UR - http://dx.doi.org/10.1021/jp952971k CP - 8 N1 - doi: 10.1021/jp952971k J1 - J. Phys. Chem. M3 - doi: 10.1021/jp952971k ER -