TY - JOUR T1 - Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and silver nanoparticles in HSC-3 cells–A mechanistic study JF - Toxicology in Vitro Y1 - 2015 A1 - Lauren A. Austin A1 - Ahmad, Samera A1 - Kang, Bin A1 - Rommel, Kathryn R A1 - Mahmoud, Mahmoud A1 - Peek, Mary E A1 - El-Sayed, Mostafa A. VL - 29 ER - TY - JOUR T1 - High-temperature surface enhanced Raman spectroscopy for in situ study of solid oxide fuel cell materials JF - Energy & Environmental Science Y1 - 2014 A1 - Li, Xiaxi A1 - Lee, Jung-Pil A1 - Blinn, Kevin S A1 - Chen, Dongchang A1 - Yoo, Seungmin A1 - Kang, Bin A1 - Bottomley, Lawrence A A1 - El-Sayed, Mostafa A. A1 - Park, Soojin A1 - Liu, Meilin VL - 7 ER - TY - JOUR T1 - Observing Real-Time Molecular Event Dynamics of Apoptosis in Living Cancer Cells using Nuclear-Targeted Plasmonically Enhanced Raman Nanoprobes JF - ACS nano Y1 - 2014 A1 - Kang, Bin A1 - Austin, Lauren A1 - El-Sayed, Mostafa A. VL - 8 SN - 1936-0851 J1 - ACS Nano ER - TY - JOUR T1 - P‐Glycoprotein‐Dependent Trafficking of Nanoparticle‐Drug Conjugates JF - Small Y1 - 2014 A1 - Dreaden, Erik C. A1 - Raji, Idris O A1 - Austin, Lauren A1 - Fathi, Shaghayegh A1 - Mwakwari, Sandra C A1 - Humphries, William H A1 - Kang, Bin A1 - Oyelere, Adegboyega K A1 - El‐Sayed, Mostafa A VL - 10 SN - 1613-6829 ER - TY - JOUR T1 - A New Nanotechnology Technique for Determining Drug Efficacy Using Targeted Plasmonically Enhanced Single Cell Imaging Spectroscopy JF - J Am Chem Soc Y1 - 2013 A1 - Austin, Lauren A1 - Kang, Bin A1 - El-Sayed, Mostafa A. AB - Recently, we described a new technique, targeted plasmonically enhanced single cell imaging spectroscopy (T-PESCIS), which exploits the plasmonic properties of gold nanoparticles, e.g. gold nanospheres, to simultaneously obtain enhanced intracellular Raman molecular spectra and enhanced Rayleigh cell scattering images throughout the entire span of a single cell cycle. In the present work, we demonstrate the use of T-PESCIS in evaluating the relative efficacy and dynamics of two popular chemotherapy drugs on human oral squamous carcinoma (HSC-3) cells. T-PESCIS revealed three plasmonically enhanced Raman scattering vibration bands, 500, 1000, and 1585 cm(-1), associated with the cellular death dynamics. Detailed analysis indicated that the decrease in the 500 cm(-1) band did not correlate well with drug efficacy but could indicate death initiation. The time it takes for the relative intensity of either the 1000 or 1585 cm(-1) band ("SERS death" bands) to appear and increase to its maximum value after the injection of a known concentration of the drug can be related to the drug's efficacy. The inverse ratio, termed cell death enhancement factor, of these characteristic death times when using either band, especially the spectrally sharp band at 1000 cm(-1), gave the correct drug efficacy ratio as determined by the commonly used XTT cell viability assay method. These results strongly suggest the potential future use of this technique in determining the efficacy, dynamics, and molecular mechanisms of various drugs against different diseases.[on SciFinder (R)] SN - 1520-5126 N1 - MEDLINE AN 2013441929(Journal; Article; (JOURNAL ARTICLE)) ER - TY - JOUR T1 - Comparative study of photothermolysis of cancer cells with nuclear-targeted or cytoplasm-targeted gold nanospheres: continuous wave or pulsed lasers JF - Journal of Biomedical Optics Y1 - 2010 A1 - Huang, Xiaohua A1 - Kang, Bin A1 - Qian, Wei A1 - Mackey, M. A. A1 - Chen, P. C. A1 - Oyelere, A. K. A1 - El Sayed, I.H. A1 - El-Sayed, Mostafa A AB - We conduct a comparative study on the efficiency and cell death pathways of continuous wave (cw) and nanosecond pulsed laser photothermal cancer therapy using gold nanospheres delivered to either the cytoplasm or nucleus of cancer cells. Cytoplasm localization is achieved using arginine-glycine-aspartate peptide modified gold nanospheres, which target integrin receptors on the cell surface and are subsequently internalized by the cells. Nuclear delivery is achieved by conjugating the gold nanospheres with nuclear localization sequence peptides originating from the simian virus. Photothermal experiments show that cell death can be induced with a single pulse of a nanosecond laser more efficiently than with a cw laser. When the cw laser is applied, gold nanospheres localized in the cytoplasm are more effective in inducing cell destruction than gold nanospheres localized at the nucleus. The opposite effect is observed when the nanosecond pulsed laser is used, suggesting that plasmonic field enhancement of the nonlinear absorption processes occurs at high localization of gold nanospheres at the nucleus. Cell death pathways are further investigated via a standard apoptosis kit to show that the cell death mechanisms depend on the type of laser used. While the cw laser induces cell death via apoptosis, the nanosecond pulsed laser leads to cell necrosis. These studies add mechanistic insight to gold nanoparticle-based photothermal therapy of cancer. (c) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3486538] VL - 15 SN - 1083-3668 N1 - Huang, Xiaohua Kang, Bin Qian, Wei Mackey, Megan A. Chen, Po C. Oyelere, Adegboyega K. El-Sayed, Ivan H. El-Sayed, Mostafa A. M3 - 10.1117/1.3486538 ER - TY - JOUR T1 - Dark-field light scattering imaging of living cancer cell component from birth through division using bioconjugated gold nanoprobes JF - Journal of Biomedical Optics Y1 - 2010 A1 - Qian, Wei A1 - Huang, Xiaohua A1 - Kang, Bin A1 - El-Sayed, Mostafa A AB - Novel methods and technologies that could extend and complement the capabilities of the prevailing fluorescence microscope in following the cell cycle under different perturbations are highly desirable in the area of biological and biomedical imaging. We report a newly designed instrument for long-term light scattering live cell imaging based on integrating a homebuilt environmental cell incubation minichamber and an angled dark-field illumination system into a conventional inverted light microscope. Peptide-conjugated gold nanoparticles that are selectively delivered to either the cytoplasmic or nuclear region of the cell are used as light scattering contrast agents. The new system enables us to carry out continuous and intermittence-free dark-field live cell imaging over several tens of hours. A variety of applications of this imaging system are demonstrated, such as monitoring the nuclear uptake of peptide-conjugated gold nanoparticles, tracking the full cycle of cancer cells from birth to division, following the chromosome dynamics during cell mitosis, and observing the intracellular distribution of gold nanoparticles after cell division. We also discuss the overall effect of nuclear targeting gold nanoparticles on the cell viability of parent and daughter cells. VL - 15 UR - http://dx.doi.org/10.1117/1.3477179 M3 - 10.1117/1.3477179 ER -